Robert Novak Our group has been studying the atmosphere of Mars using CSHELL since 1997. Our first studies measured O2(1g) emissions (a tracer of ozone), and HDO absorptions (then a proxy for water; we now measure both HDO and H2O to measure D/H). By placing the spectrograph slit across the planet N/S, we were able to obtain latitudinal maps of these species; by stepping the slit across the planet, we obtained 2-D maps over the entire range of Martian season. These initial measurements led to further studies such as the geographic and diurnal variation of the D/H ratio of Mars’ water and the identification of methane on Mars. We began using ISHELL to study Mars in January, 2017. ISHELL with its higher spectral resolution (70000 vs 40000) and its wider spectral range per image (~ 200 times larger) enhances the quality of data taken with CSHELL, and enables a simultaneous search for several related species (ad always CO2). With a larger spectral grasp per image, more spectral lines per species can be measured resulting in more precise measurements. Likewise, more molecules may be measured simultaneously without changing the spectral setting of the instrument. Our previous measurements of the D/H ratio required two different spectral settings; both can now be measured in one setting. When we measured methane, one setting using CSHELL gave us one absorption line. An ISHELL setting give us an entire band plus bands for related species such as ethane, propane, and butane. From measuring these organic species simultaneously, we can test whether the methane is of geological or biological origin. In summary, using ISHELL to observe Mars’ atmosphere will provide enhanced measurements of molecules that have been already measured and will enable measurements of molecules that have not yet been detected on Mars, especially those that have astrobiological significance. -R. Novak, M. Mumma, G. Villanueva, S. FAggi